Murine erythroleukaemia cells (Friend cells) possess high-affinity binding sites for erythropoietin

Spermine induces haemoglobin synthesis in murine erythroleukaemia cells.

The naturally occurring polyamine spermine induces haemoglobin synthesis in murine erythroleukaemia (MEL) cells. Haemoglobin production was accompanied by accumulation of cytoplasmic beta-globin mRNA and growth inhibition, but not by cell-cycle block or changes in cell volume. Hexamethylene-bisacetamide (HMBA), a well known differentiating agent, also induces haemoglobin production, but causes ...

Non-polyadenylated messenger ribonucleic acids with affinity for polyadenylyl-Sepharose from Friend murine leukaemia cells.

The existence of a class of eukaryotic mRNA with a 3’-terminal sequence of poly(A) is now well established (Darnel1 et al., 1973; Greenberg, 1975; Brawerman, 1976). It is now clear that not all mRNA species have this structural feature. In particular, histone messengers were shown to lack poly(A) (Adesnick & Darnell, 1972; Greenberg & Perry, 1972) and more recently other non-polyadenylated mess...

Erythropoietin activates the receptor in both Rauscher and Friend murine erythroleukemia cells.

Alterations in the expression of two proto-oncogenes, c-myb and c-myc, have been implicated in the differentiation of transformed erythroid cells induced by chemical inducers, such as dimethyl sulfoxide (Me2SO). In the present study, we compared the expression of c-myb and c-myc during erythropoietin (Epo) and Me2SO induction of Rauscher erythroleukemia cells, which differentiate in response to...

Reversibility of high-affinity binding of methotrexate in L1210 murine leukemia cells.

Binding and receptor-mediated endocytosis of erythropoietin in Friend virus-infected erythroid cells.

The binding of labeled erythropoietin (EP) to cell surface receptors and subsequent processing of the hormone within the cell was studied in erythroid cells procured from the spleens of mice infected with the anemia strain of Friend virus. These immature erythroid cells respond to EP in culture to differentiate into reticulocytes and erythrocytes. Radiolabeled EP (both iodinated and tritiated) ...